RESEARCH OF ACTIVITY AND PHYSICAL-CHEMICAL PROPERTIES OF CATHEPSINE D IN DRONE BROOD LARVAE AT DIFFERENT STAGES OF DEVELOPMENT 

Grishina Zhanna Waler’evna, Postrgraduate student, Penza State University (40 Krasnaya street, Penza, Russia), grinzanetk@gmail.com
Gengin Mikhail Trofimovich, Doctor of biological sciences, professor, sub-department of biology and biochemistry, Penza State University (40 Krasnaya street, Penza, Russia), gengin07@yandex.ru

577.12

10.21685/2307-9150-2016-4-2

Background. In our study, we examined the activity and some physicochemical properties of cathepsin D in drone brood larvae at different stages of ontogeny in order to clarify the dynamics of changes in the activity of cathepsin D and its role in the development of drone larvae.
Materials and methods. The dealt with drone brood larvae of various ages. By gel filtration the authors identified two enzyme forms, differing in molecular weight. The researchers found the pH optimum of action of these forms and studied their relation to a specific inhibitor of cathepsin D – pepstatin.
Results. The authors ascertained the dynamics of changes of cathepsin D activity in the larvae of drone brood at different stages of development. It identified two forms, differing in the molecular weight of the enzyme in the larvae of drone brood. It was found that a specific inhibitor of cathepsin D of vertebrates – pepstatin – is ineffective for cathepsin D of drone larvae.
Conclusions. It is expected that such dynamics of cathepsin D activity changes partially reflects the metabolism of proteins in a cell at a particular stage of larval development.

cathepsine D, drone brood larvae, ontogenesis

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